@akorvemaker : If your Yubikey is the single-factor type, it won't work beginning today. From today's blog post: "A few rarely used types of alternative login were discontinued today. If you had one of these set up, you will no longer be able to use it to log in. You will need to use your master password. The affected types are: ... Yubikey one factor (no base password) ..."
The issue with the trust-this-computer checkbox... I've seen this same problem on other sites. A great solution is to either leave it unchecked by default (as others have said above), or have a browser cookie set to remember the unchecked state from an earlier session.
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